Affinity chromatography of thiol-containing purines and ribonucleic acid.
Biochem J
; 168(3): 595-7, 1977 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-606258
ABSTRACT
Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tionucleosídeos
/
RNA
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1977
Tipo de documento:
Article