[Isolation and properties of an alpha-galactosidase preparation from Cephalosporium sp. 237]. / Poluchenie i svoistva preparata al'fa-galaktozidazy iz Cephalosporium sp. 237.
Prikl Biokhim Mikrobiol
; 18(3): 401-4, 1982.
Article
em Ru
| MEDLINE
| ID: mdl-6285332
ABSTRACT
Different methods for preparing alpha-galactosidase from the culture fluid of the micromycete Cephalosporium sp. 237 were tested. They included precipitation with ethanol, isopropanol, and acetone. Precipitation with two volumes of acetone gave the best results with respect to specific activity (12 units) and yield of the enzyme (78%). Properties of alpha-galactosidase (optimum pH at 5.5, temperature optimum at 40 degrees C. thermolability, etc), when different substrates were used, were examined.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acremonium
/
Alfa-Galactosidase
/
Galactosidases
Idioma:
Ru
Revista:
Prikl Biokhim Mikrobiol
Ano de publicação:
1982
Tipo de documento:
Article