Similar rate of O6-ethylguanine elimination from DNA in normal human fibroblast and xeroderma pigmentosum cell strains not transformed by SV40.
Carcinogenesis
; 4(8): 1075-7, 1983 Aug.
Article
em En
| MEDLINE
| ID: mdl-6307543
Using a highly specific rabbit antiserum (E3) directed against O6-ethyldeoxyguanosine in a competitive radioimmunoassay, we have measured the kinetics of removal of this alkylation product from the DNA of human fibroblasts treated with the N-nitroso carcinogen N-ethyl-N-nitrosourea. A very similar rate of elimination from DNA was found in two normal human fibroblast strains (GM730 and 54BR) and in the nonvirus-transformed xeroderma pigmentosum fibroblast strains XP2BI and XP3BR. This is in contrast to the SV40-transformed xeroderma pigmentosum fibroblast strain XP12RO-SV40 which had previously been shown to be defective in its ability to remove O6-ethylguanine from DNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xeroderma Pigmentoso
/
DNA
/
Transformação Celular Neoplásica
/
Vírus 40 dos Símios
/
Guanina
Limite:
Humans
Idioma:
En
Revista:
Carcinogenesis
Ano de publicação:
1983
Tipo de documento:
Article