Cloning of eukaryotic genes in single-strand phage vectors: the human interferon genes.
Gene
; 27(1): 87-99, 1984 Jan.
Article
em En
| MEDLINE
| ID: mdl-6325303
ABSTRACT
Using oligonucleotide probes with defined sequences, we have selected clones from a human lymphocyte cDNA library which represent human leukocyte (HuIFN-alpha) and fibroblast (HuIFN-beta) interferon gene sequences. Double-stranded f1 phage DNA was used as the vector for initial cloning of cDNA. Clones carrying interferon gene sequences were identified by hybridization with the oligonucleotide probes. The same oligonucleotide probes were used as primers for dideoxy chain termination sequencing of the clones. One HuIFN-alpha clone, 201, has a nucleotide sequence different from published HuIFN-alpha sequences. Under control of the lacUV5 promoter, the 201 gene has been used to express biologically active HuIFN-alpha in Escherichia coli.
Buscar no Google
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Interferon Tipo I
/
Vetores Genéticos
Limite:
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1984
Tipo de documento:
Article