Incorporation in liposomes as a method for the application of genotoxins of low water solubility in the SCE assay.
Mutat Res
; 139(4): 207-10, 1984 Apr.
Article
em En
| MEDLINE
| ID: mdl-6325903
ABSTRACT
Benzo[alpha]pyrene BaP), freely dissolved or incorporated in liposomes prepared from egg yolk lecithin, was checked for SCE induction in Chinese hamster V79-E and rat liver RL-19 cells in vitro. SCE induction in V79-E was observed only when freely dissolved BaP was added together with S9 mix. RL-19 cells were per se highly sensitive to SCE induction by BaP either freely dissolved or incorporated in liposomes. It is suggested that the incorporation of genotoxins in liposomes is a practicable method for the application, in mammalian genotoxicity assays, of agents which are barely soluble or completely insoluble in water, provided no exogenous metabolizing system is required.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Troca de Cromátide Irmã
/
Benzopirenos
/
Troca Genética
/
Lipossomos
/
Mutagênicos
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1984
Tipo de documento:
Article