On the phospholipid metabolism of glial cell primary cultures. III. Utilization of 1-alkenyl-glycerophosphoethanolamine (lysoplasmalogen).

ABSTRACT

Primary cultures prepared from newborn rat brain, consisted after 16 or 17 days mainly of astrocytes and of oligodendrocytes. 1-Alkenyl-sn-glycero-3-phosphoethanolamine (lysoplasmalogen) was used as substrate for studies on the metabolism of ethanolamine-glycerophospholipids. After 3 hr incubation two main products were observed a) 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine (= ethanolamine plasmalogen) and b) 1-alkenyl-2-acyl-sn-glycero-3-phosphocholine (= choline plasmalogen). The acylation rate reached saturation at about 10 nmol substrate/mg cell protein with a Vmax of 30 nmol x mg cell protein-1 x 3 hr-1. This acylated compound amounted to almost 60% of all radioactivity internalized, whereas the second product, choline plasmalogen, came to 20%. Unchanged substrate was found within the cells only in small amounts, even at maximum substrate internalization. These results were discussed in comparison with those obtained with 1-alkyl-sn-glycero-3-phosphoethanolamine under the same conditions (25).