[Chromosome aberrations in human lymphocytes induced by exposure to different doses of chronic gamma irradiation in vitro]. / Khromosomnye aberratsii v limfotsitakh cheloveka, vyzvannye vozdeistviem razlichnykh doz khronicheskogo gamma-oblucheniia in vitro.

ABSTRACT

Non-stimulated with phytohemagglutinin human blood was chronically gamma-irradiated (226Ra, 99 mg) in vitro at 37 derees C. The dose rates were 8.2 (irradiation doses 8.2, 16.4, 41 and 162.2 rads) and 25.3 (irradiation doses 8.4, 16.9, 42.2, 168.2, 337.3 and 506 rads) rads/hour. Quantitative analysis of different types of structural chromosome aberrations was carried out. The data on aberrant cells and dicentrics were statistically interpreted. The frequency of aberrant cells, observed at both dose rates, was statistically described by the equation y = a + bD, where y = 7.11 (+/- 0.56) + 11.38 (+/- 2.32) . 10(-2) D, and y = 5.23 (+/- 0.23 + 13.20 (+/- 1.11) . 10(-2) D at dose rates 8.2 and 25.3 rads respectively. The dicentrics yield was described by the equation y = alphaD + betaD2 + C, where y = 3.19 (+/- 0.22) . 10(-2) D + 7.0 (+/- 0.46) . 10(-5) D2 + 0.42, and y = 5.05 (+/- 0.38) . 10(-2) D + 7.1 (+/- 0.41) . 10(-5) D2 + 0.42 at 8.2 and 25.3 dose rates. Statistically reliable differences in the dicentric yield in lymphocytes irradiated with different dose rates are observed. The results obtained show that the increase in the dose rate results in the increase of the dicentric yield, originated in one-track process, while the dicentric yield from two-track process remains constant.