Determination of 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (virazole) in blood and urine by chemical ionization-mass fragmentography.
J Chromatogr
; 160(1): 169-79, 1978 Oct 11.
Article
em En
| MEDLINE
| ID: mdl-730788
ABSTRACT
A combined gas chromatographic-mass spectrometric technique is described for the quantification of virazole in serum and urine. Proteins are removed by molecular filtration, lipids by extraction with dichloromethane and interfering endogenous constituents by acidic and basic ion-exchange resins. Virazole is quantified by monitoring the protonated molecular ions of the fully silylated derivatives of virazole (m/e 533) and the arabinose analog (internal standard) obtained by methane chemical ionization. The detection limit is 150 pg (0.6.10(-12) mole) of virazole injected. In serum 10 ng/ml (4.10(-8) mole) can be detected, 25 ng/ml quantified. In urine 0.5 microgram/ml can be quantified without preconcentration. Virazole was detected in serum for at least 96 h at the 70-ng/ml level.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribavirina
/
Ribonucleosídeos
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1978
Tipo de documento:
Article