Sepsis-induced acute lung injury is attenuated by selectin blockade following the onset of sepsis.
Arch Surg
; 130(11): 1199-208, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-7487463
ABSTRACT
OBJECTIVE:
To determine the effect of infusion with a dual-binding antibody to E- and L-selectin, EL-246, in a postonset model of sepsis.DESIGN:
Nonrandomized controlled study. STUDYSUBJECTS:
Young Yorkshire swine.INTERVENTIONS:
Three groups were studied. Controls (n = 8) received saline solution only. Untreated animals with sepsis (n = 8) received a 1-hour intravenous infusion of live Pseudomonas aeruginosa. Animals treated with EL-246 (n = 6) received the same bacterial infusion and a 2-mg/kg bolus of EL-246 at 30 minutes. OUTCOMEMEASURES:
Systemic and pulmonary hemodynamics, arterial blood gas determination, bronchoalveolar lavage protein and neutrophil content, neutrophil integrin and selectin expression, neutrophil oxidant burst, and organ myeloperoxidase content.RESULTS:
Treatment with EL-246 significantly reduced lung injury, as indicated by improved bronchoalveolar lavage protein and neutrophil content, resulting in a significant improvement in arterial oxygenation. This reduction in lung injury was produced by a reduction in lung myeloperoxidase content. Treatment with EL-246 failed to prevent the development of pulmonary hypertension and systemic hypotension. Neutrophils from animals with sepsis exhibited significant activation and upregulation of CD18, shedding of L-selectin, and production of increased levels of oxidants compared with controls.CONCLUSION:
Treatment of animals with EL-246 soon the onset of sepsis produced significant protection against acute lung injury but failed to attenuate hemodynamic derangements associated with sepsis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sepse
/
Selectinas
/
Pneumopatias
/
Anticorpos
Limite:
Animals
Idioma:
En
Revista:
Arch Surg
Ano de publicação:
1995
Tipo de documento:
Article