Pathogenesis of foot-and-mouth disease in swine, studied by in-situ hybridization.
J Comp Pathol
; 113(1): 51-8, 1995 Jul.
Article
em En
| MEDLINE
| ID: mdl-7490337
ABSTRACT
Eight 7-month-old pigs were inoculated intradermally with 10(3) plaque-forming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence of virus and its correlation with lesion development. The probe for in-situ hybridization was a biotinylated 500-base negative-sense transcription product corresponding to a portion of the gene encoding polymerase. With this technique, virus was shown to be widely disseminated in all epidermal tissues, regardless of histologically apparent cellular disruption.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Hibridização In Situ
/
Febre Aftosa
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Comp Pathol
Ano de publicação:
1995
Tipo de documento:
Article