Molecular cloning of the testicular follicle stimulating hormone receptor of the non human primate Macaca fascicularis and identification of multiple transcripts in the testis.
Biochem Biophys Res Commun
; 196(3): 1066-72, 1993 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-7504463
ABSTRACT
Reverse transcription PCR was used to amplify the complete open reading frame of the follicle-stimulating hormone receptor (FSHR) from testicular poly (A)+ RNA of the non-human primate Macaca fascicularis. Along with the structural motifs of a G-protein coupled receptor, sequence analysis reveals that the monkey FSHR is highly homologous to the human FSHR and has specific features such as N-linked glycosylation sites which are identical to the human FSHR but not present in the rat or ovine FSHR. Northern blot hybridization of testicular poly (A)+ RNA to a cRNA probe corresponding to the extracellular domain of the monkey FSHR resulted in the identification of several transcripts, indicating alternative splicing events of the primary transcript.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Receptores do FSH
/
Macaca fascicularis
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1993
Tipo de documento:
Article