Comparison of the positional cloning methods used to isolate the BRCA1 gene.
Hum Mol Genet
; 4(8): 1259-66, 1995 Aug.
Article
em En
| MEDLINE
| ID: mdl-7581362
ABSTRACT
A critical step in positional cloning is the identification of candidate genes from a large, genetically defined region. Candidate gene isolation by hybrid selection, genomic sequencing, and direct cDNA library screening identified 45 candidate gene fragments (CGFs) from a 600 kb genomic region that contains the BRCA1 gene. These CGFs define a minimum of 15 genes, six of which are newly localized to the BRCA1 region. We present an analysis of the efficiency and the sequences generated for each of these methods. We also compare our CGF set to those reported for the BRCA1 region by three other groups, revealing a surprising lack of overlap among the sets.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oncogenes
/
Neoplasias da Mama
/
Clonagem Molecular
Limite:
Female
/
Humans
Idioma:
En
Revista:
Hum Mol Genet
Ano de publicação:
1995
Tipo de documento:
Article