Exogenous transforming growth factor-beta amplifies its own expression and induces scar formation in a model of human fetal skin repair.
Ann Surg
; 222(2): 146-54, 1995 Aug.
Article
em En
| MEDLINE
| ID: mdl-7639582
ABSTRACT
OBJECTIVE:
Fetal skin wounds heal without scarring. To determine the role of TGF-beta 1 in fetal wound healing, mRNA expression of TGF-beta 1 was analyzed in human fetal and adult skin wounds.METHODS:
Human fetal skin transplanted to a subcutaneous location on an adult athymic mouse that was subsequently wounded heals without scar, whereas human adult skin heals with scar formation in that location. In situ hybridization for TGF-beta 1 mRNA expression and species-specific immunohistochemistry for fibroblasts, macrophages, and neutrophils were performed in human adult wounds, fetal wounds, and fetal wounds treated with a TGF-beta 1 slow release disk.RESULTS:
Transforming growth factor-beta 1 mRNA expression was induced by wounding adult skin. No TGF-beta 1 mRNA upregulation was detected in human fetal skin after wounding. However, when exogenous TGF-beta 1 was added to human fetal skin, induction of TGF-beta 1 mRNA expression in human fetal fibroblasts occurred, an adult-like inflammatory response was detected, and the skin healed with scar formation.CONCLUSIONS:
Transforming growth factor-beta 1 is an important modulator in scar formation. Anti-TGF-beta 1 strategies may promote scarless healing in adult wounds.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Fator de Crescimento Transformador beta
/
Cicatriz
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Feto
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Revista:
Ann Surg
Ano de publicação:
1995
Tipo de documento:
Article