Effects of gamma radiation on the OM431 human ocular melanoma cell line.
Exp Eye Res
; 60(6): 603-5, 1995 Jun.
Article
em En
| MEDLINE
| ID: mdl-7641843
ABSTRACT
In order to determine the dose responsiveness to radiation of ocular melanoma, we conducted an in vitro dose-response study on a monolayer cell culture using a clonogenic assay. The effects on cell survival were determined relative to unirradiated controls. A human epithelioid ocular melanoma cell line, OM431, was maintained in tissue culture and serial dilutions of viable cells were plated in flasks, allowed to settle and attach for 48 h, and subsequently irradiated with 1-10 Gy in single fractions. After 2 weeks, the number of reproducing clones (forming colonies with greater than 32 cells or five generations) were counted. The surviving fractions of cells were plotted on a cell survival curve using the linear quadratic model. The survival curve showed a large initial shoulder followed by an exponential decline in growth. Our data suggest that the OM431 ocular melanoma cell line responds to irradiation in a manner similar to other melanoma cell lines and is relatively radioresistant especially at lower doses.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Oculares
/
Raios gama
/
Melanoma
Limite:
Humans
Idioma:
En
Revista:
Exp Eye Res
Ano de publicação:
1995
Tipo de documento:
Article