Cyclic amphipathic peptide-DNA complexes mediate high-efficiency transfection of adherent mammalian cells.
Proc Natl Acad Sci U S A
; 90(3): 893-7, 1993 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-7679217
ABSTRACT
A DNA transfection protocol has been developed that makes use of the cyclic cationic amphipathic peptide gramicidin S and dioleoyl phosphatidylethanolamine. The DNA complex is formed by mixing gramicidin S with DNA at a 11 charge ratio and then adding phosphatidylethanolamine at a lipid/peptide molar ratio of 51. The complex mediates rapid association of DNA with cells and leads to transient expression levels of beta-galactosidase ranging from 1 to 30% of the transfected cells, with long-term expression being about an order of magnitude lower. The respective roles of peptide and phospholipid are not yet resolved but optimal transfection requires both the cyclic peptide and the hexagonal phase-competent phospholipid PtdEtn. Transfection in CV-1 cells is not affected by lysomotrophic agents, which suggests that DNA entry into the cell is via the plasma membrane. This technique that is simple, economical, and reproducible mediates transfection levels up to 20-fold higher than cationic liposomes in adherent mammalian cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
DNA
/
Transfecção
/
Lipossomos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1993
Tipo de documento:
Article