[A new two-stage plan for isolating human alpha1-acidic glycoprotein (orosomucoid). Orosomucoid interacts with ethidium bromide]. / Novaia dvukhstadiinaia skhema vydeleniia chelovecheskogo alpha1- kislogo glikoproteina (orozomukoida). Orozomukoid vzaimodeistvuet s bromistym étidiem.
Bioorg Khim
; 20(12): 1297-309, 1994 Dec.
Article
em Ru
| MEDLINE
| ID: mdl-7695648
ABSTRACT
Human alpha 1-acid glycoprotein (orosomucoid, AGP) was purified to homogeneity by a two-step procedure using phenol and chloroform deproteinization of serum with subsequent preparative electrophoresis on agarose gel. It was obtained 0.15-0.3 mg of protein from 1 ml of serum. Human alpha 1-AGP binds ethidium bromide but not DNA. Neither other human serum proteins nor mice and rat alpha 1-AGP bind ethidium bromide. This property of human orosomucoid depends on its amino acid sequence rather than on the carbohydrate part of the molecule.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Orosomucoide
/
Etídio
Limite:
Female
/
Humans
/
Male
Idioma:
Ru
Revista:
Bioorg Khim
Ano de publicação:
1994
Tipo de documento:
Article