A cell-free protein translocation system prepared entirely from a gram-positive organism.
FEBS Lett
; 362(1): 29-33, 1995 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-7698347
ABSTRACT
A cell-free protein translocation system derived exclusively from a Gram-positive bacterium is described here for the first time. Highly efficient in vitro synthesis of plasmid encoded preprolipase of Staphylococcus hyicus is accomplished by coupled transcription/translation using either a cytosolic extract of S. carnosus alone or in combination with T7-RNA-polymerase. Addition of inside-out cytoplasmic membrane vesicles of S. carnosus leads to the partial conversion (processing) of preprolipase to prolipase. In addition, as shown in a protease protection assay, a significant part of preprolipase plus prolipase is translocated in vitro into the lumen of the vesicles. Translocation of preprolipase into the membrane vesicles requires the proton-motive force and the S. carnosus SecA protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Staphylococcus
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Proteínas de Bactérias
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Membrana Celular
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Adenosina Trifosfatases
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Proteínas de Escherichia coli
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Precursores Enzimáticos
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Lipase
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1995
Tipo de documento:
Article