[Replacement of native sera with growth-stimulating proteins during culturing of mammalian cells]. / Zamena nativnykh syvorotok roststimuliruiushchimi belkami pri kul'tivirovanii kletok mlekopitaiushchikh.
Vopr Virusol
; 40(1): 39-41, 1995.
Article
em Ru
| MEDLINE
| ID: mdl-7740788
The authors recommend to replace nonstandard native animal sera by growth-stimulating proteins isolated from the sera of cattle, northern deer, pigs, and sheep by fractionation with polyethylene glycol aqueous solutions (mol. m 6000 dalton). Growth-stimulating proteins are conducive to active proliferation of primary and continuous cell cultures at various methods of culturing. Growth-stimulating proteins were for the first time added to nutrient media during culturing of mammalian cells on microcarriers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sangue
/
Proteínas
/
Substâncias de Crescimento
Limite:
Animals
/
Humans
Idioma:
Ru
Revista:
Vopr Virusol
Ano de publicação:
1995
Tipo de documento:
Article