Macrophage requirement for the in vitro response to TNP Ficoll: a thymic independent antigen.
J Immunol
; 116(6): 1579-81, 1976 Jun.
Article
em En
| MEDLINE
| ID: mdl-774987
ABSTRACT
The in vitro antibody-forming cell response of mouse spleen cells to the thymic independent synthetic polymer, TNP-Ficoll, was found to require the presence of either macrophages or 2-mercaptoethanol. The TNP-Ficoll response and that to the thymic dependent antigen, sheep erythrocytes, demonstrated the same degree of macrophage dependence. However, macrophage-depleted spleen cells which did not produce plaque-forming cells responded normally to T and B cell mitogens. Comparison of the antibody-forming cell response of macrophage-depleted spleen cells in the presence and absence of 2-mercaptoethanol indicated that either macrophages function 10 to 100 times more efficiently in its presence or that 2-mercaptoethanol partially replaces the function of macrophages.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Ficoll
/
Macrófagos
/
Formação de Anticorpos
/
Nitrobenzenos
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1976
Tipo de documento:
Article