Requirement for phosphatidylinositol transfer protein in epidermal growth factor signaling.
Science
; 268(5214): 1188-90, 1995 May 26.
Article
em En
| MEDLINE
| ID: mdl-7761838
ABSTRACT
Stimulation of phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis is a widespread mechanism for receptor-mediated signaling in eukaryotes. Cytosolic phosphatidylinositol transfer protein (PITP) is necessary for guanosine triphosphate (GTP)-dependent hydrolysis of PIP2 by phospholipase C-beta (PLC-beta), but the role of PITP is unclear. Stimulation of phospholipase C-gamma (PLC-gamma) in A431 human epidermoid carcinoma cells treated with epidermal growth factor (EGF) required PITP. Stimulation of PI-4 kinase in cells treated with EGF also required PITP. Coprecipitation studies revealed an EGF-dependent association of PITP with the EGF receptor, with PI-4 kinase, and with PLC-gamma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Proteínas de Transporte
/
Fator de Crescimento Epidérmico
/
Proteínas de Membrana
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1995
Tipo de documento:
Article