Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride.
Electrophoresis
; 15(7): 930-1, 1994 Jul.
Article
em En
| MEDLINE
| ID: mdl-7813397
ABSTRACT
We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate and methanol concentrations in the preequilibration washes and length of electrotransfer. We demonstrated that the method is useful for tubulin, a protein that resists usual semi-dry transfer procedures due to its limited solubility under IEF conditions. We also successfully used our method for transferring the microtubule-associated protein, tau, as well as carbamylyte standards, creatine phosphokinase and carbonic anhydrase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polivinil
/
Resinas Acrílicas
/
Eletroforese em Gel de Poliacrilamida
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Géis
/
Focalização Isoelétrica
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
1994
Tipo de documento:
Article