Optical purity determination of thyroxine enantiomers in bulk materials by chiral thin layer chromatography.

ABSTRACT

L-thyroxine and D-thyroxine were separated on ligand exchange chiral thin layer chromatographic plates, using a solvent system consisting of acetonitrilemethanolwater 601515 v/v, at a wavelength of 254 nm. The methodology, chiral recognition mechanism(s) involved and its application are discussed.