Lectin electron microscopic histochemistry of the pseudoexfoliative material in the skin.
Invest Ophthalmol Vis Sci
; 35(11): 3962-6, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7928196
ABSTRACT
PURPOSE:
To investigate the hypothesis that pseudoexfoliation (PSX) syndrome is a systemic disorder, the authors studied the composition of glycoconjugates in the intraocular and extraocular PSX material at the electron microscopic level, using a panel of lectins as cytochemical probes.METHODS:
The authors examined 8 lid skins, 11 trabecular tissues, and 3 cataractous lenses from human eyes with PSX syndrome. Tissues were processed for electron microscopical histochemistry and stained with PNA, RCA120, DBA, SBA, ConA, WGA, UEA-I, and Lotus, with an indirect lectin-colloidal gold technique.RESULTS:
Both the intraocular and extraocular PSX materials manifested almost identical reactivity to lectins, which indicated that glycoconjugates in the PSX material contained with sugar residues of galactose (PNA, RCA120), alpha-mannose (ConA), and N-acetyl-D-glucosamine (WGA). On the other hand, it was indicated that sugar residues of N-acetyl-D-galactosamine (DBA, SBA) and fucose (UEA-I, Lotus) were absent. Granular inclusions and microfibrils in the capsule and ocular zonules were stained similarly and weakly.CONCLUSIONS:
The intraocular and extraocular PSX materials contained the same sugar residues of glycoconjugates, which suggested that those materials had the same nature. This study, the first documentation of lectin-binding sites on the extraocular PSX material, supported the hypothesis of PSX syndrome as a systemic disorder.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Glicoconjugados
/
Síndrome de Exfoliação
Limite:
Humans
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
1994
Tipo de documento:
Article