Characterization of the gcv control region from Escherichia coli.
J Bacteriol
; 176(20): 6159-64, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7928983
ABSTRACT
We constructed a set of deletions upstream of the gcv promoter and analyzed the effects of the deletions on expression of a gcvT-lacZ gene fusion. A deletion that ends at position -313 upstream of the transcription initiation site (+1) results in reduced levels of gcvT-lacZ expression, but the fusion is still inducible by glycine and repressible by purines. A deletion that ends at position -169 results in loss of both GcvA- and Lrp-mediated activation of the gcvT-lacZ fusion. The endpoints of delta -313 and delta -169 also define a site that down-regulates gcvT-lacZ expression two- to threefold. A deletion that ends at position -89 upstream from the transcription initiation site still shows PurR-mediated repression, suggesting that PurR-mediated repression is not by direct interference with the GcvA- and Lrp-mediated regulatory mechanism(s). Gel mobility shift assays and DNase I footprinting showed that Lrp protein binds to multiple sites upstream of the gcv promoter, from about bp -92 to bp -229. The results suggest that the gcv regulatory region is complex, with numerous cis-acting sites that are required for normal gcv expression.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Transferases
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Proteínas de Transporte
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Regulação Enzimológica da Expressão Gênica
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Sequências Reguladoras de Ácido Nucleico
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Hidroximetil e Formil Transferases
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Escherichia coli
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Aminoácido Oxirredutases
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Complexos Multienzimáticos
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1994
Tipo de documento:
Article