Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.
Nature
; 372(6505): 432-8, 1994 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-7984237
ABSTRACT
The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the surface of the beta-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Nuclear Pequeno
/
Proteínas de Ligação a RNA
/
Spliceossomos
/
Ribonucleoproteína Nuclear Pequena U1
Idioma:
En
Revista:
Nature
Ano de publicação:
1994
Tipo de documento:
Article