Oxidative stress-induced apoptosis prevented by Trolox.
Free Radic Biol Med
; 16(6): 675-84, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-8070670
ABSTRACT
The ability of oxidative stress to induce apoptosis (programmed cell death), and the effect of Trolox, a water soluble vitamin E analog, on this induction were studied in vitro in mouse thymocytes. Cells were exposed to oxidative stress by treating them with 0.5-10 microM hydrogen peroxide (H2O2) for 10 min, in phosphate-buffered saline supplemented with 0.1 mM ferrous sulfate. Cells were resuspended in RPMI 1640 medium with 10% serum and incubated at 37 degrees C under 5% CO2 in air. Electron microscopic studies revealed morphological changes characteristic of apoptosis in H2O2-treated cells. H2O2 treatment fragmented the DNA in a manner typical of apoptotic cells, producing a ladder pattern of 200 base pair increments upon agarose gel electrophoresis. The percentage of DNA fragmentation (determined fluorometrically) increased with increasing doses of H2O2 and postexposure incubation times. Pre- or posttreatment of cells with Trolox reduced H2O2-induced DNA fragmentation to control levels and below. The results indicate that oxidative stress induces apoptosis in thymocytes, and this induction can be prevented by Trolox, a powerful inhibitor of membrane damage.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Cromanos
/
Apoptose
/
Peróxido de Hidrogênio
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Free Radic Biol Med
Ano de publicação:
1994
Tipo de documento:
Article