Synchronous calcium oscillations in cerebellar granule cells in culture mediated by NMDA receptors.
Neuroreport
; 4(5): 539-42, 1993 May.
Article
em En
| MEDLINE
| ID: mdl-8099816
ABSTRACT
The concentration of cytosolic Ca2+ ([Ca2+]i) was monitored in cerebellar granule cell cultures by digital imaging of fura-2 loaded cells. In the presence of Mg2+, cells grown in low K+ cultures responded to N-methyl-D-aspartate (NMDA) with uniform increases in [Ca2+]i from a stable basal [Ca2+]i. In contrast, in Mg(2+)-free medium, low K+ cultures showed spontaneous, synchronized [Ca2+]i oscillations from 4 days in culture. The oscillations were rapidly blocked by Mg2+, D,L-2-amino-5-phosphonovalerate, or tetrodotoxin. The development of oscillatory behaviour depended on the culture conditions and was not observed in cultures grown in high K+. These data show a high degree of connectivity established within 4 days in culture by dissociated granule cells allowing synchronized activity mediated through synaptic mechanisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cerebelo
/
Cálcio
/
Receptores de N-Metil-D-Aspartato
/
Grânulos Citoplasmáticos
Limite:
Animals
Idioma:
En
Revista:
Neuroreport
Ano de publicação:
1993
Tipo de documento:
Article