Short-term primary cultures in studies of post-natal maturation of the rat proximal tubule-proton and bicarbonate transport.
Pediatr Nephrol
; 7(6): 798-801, 1993 Dec.
Article
em En
| MEDLINE
| ID: mdl-8130112
ABSTRACT
This is a review of recent work based on an in vitro model which has allowed us to investigate the postnatal maturation of renal epithelial cells. Renal proximal tubule cells from 8- to 40-day-old Sprague-Dawley rats were studied after 48 h of primary culture. The regulation of intracellular pH (pHi) was measured by quantitative fluorescence microscopy using 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Recordings were made under basal conditions and after imposing a cytoplasmic alkalosis or acidosis using 15 mM ammonium salt. The ability of the cells to recover from both acidosis and alkalosis improved during post-natal maturation. The improvement in recovery from intracellular acidosis could be entirely accounted for by an increase in the rate of Na+/H+ exchange. The capacity for Na+/H+ exchange was independent of the cellular growth rate, but depended on cellular differentiation. A developmental increase in the activity of Cl-/HCO3- exchange between 12 and 14 days of age was also demonstrated. No developmental change was seen in either steady-state pHi (7.27-7.35) or in cytoplasmic buffer capacity (37.6-44.4 mM/pHi). The characteristics of transporter maturation revealed by these experiments are very similar to those observed in isolated perfused proximal tubules of developing rabbits.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bicarbonatos
/
Proteínas de Transporte
/
Trocadores de Sódio-Hidrogênio
/
Antiporters
/
Túbulos Renais Proximais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Pediatr Nephrol
Ano de publicação:
1993
Tipo de documento:
Article