Intracerebroventricular administration of canatoxin to rats increases the circulating levels of luteinizing hormone.
Braz J Med Biol Res
; 26(11): 1231-5, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8136723
ABSTRACT
Recent evidence has implicated the central nervous system as a target organ for canatoxin, a toxic protein present in Canavalia ensiformis seeds. This toxin activates the lipoxygenase pathway of arachidonic acid metabolism and can thus induce the release of substances mediated by lipoxygenase products. In the present study, the circulating levels of luteinizing hormone (LH) were measured by RIA in male Wistar rats (200-240 g) after the administration of canatoxin into the lateral cerebral ventricle. Canatoxin (0.5-2 micrograms in 2 microliters daily for 3 days) caused a dose- and time-dependent increase in the plasma levels of LH. The total dose of canatoxin used is subconvulsive. At 2, 4 and 24 h after 2 micrograms of canatoxin LH levels were increased by 10%, 43% and 61%, respectively, compared to vehicle-injected animals (0.18 +/- 0.03 ng/ml). This response to 2 micrograms of canatoxin was not attenuated by pretreatment with two different lipoxygenase inhibitors, nordihydroguaiaretic acid (NDGA, 125 mg/kg) or esculetin (ECLT, 125 mg/kg), ip, 1 h before each canatoxin (CNTX) injection; % increase in LH with CNTX alone 61%; CNTX+NDGA 54%; CNTX+ECLT76%; N = 5/group. These data show that intracerebral injection of CNTX in rats increases circulating levels of LH via a mechanism that is independent of the lipoxygenase pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Toxinas Biológicas
/
Hormônio Luteinizante
/
Lectinas
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
1993
Tipo de documento:
Article