Practical use of polymerase chain reaction for the diagnosis of steroid induced tuberculous lymphadenitis.
J Trop Med Hyg
; 97(2): 65-8, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-8170004
ABSTRACT
Tuberculous lymphadenitis that had developed after treatment with systemic prednisolone was diagnosed using the polymerase chain reaction (PCR) combined with dot-blot hybridization. Although the patient had no history of tuberculosis or suggestive radiological findings, a DNA fragment specific for Mycobacterium tuberculosis complex strains was amplified from a paraffin-embedded biopsy specimen of a cervical swelling, which was compatible with tuberculous lymphadenitis on haematoxylin-eosin (HE) and Ziehl-Neelsen staining. This report demonstrates the practical use of PCR for diagnosis of extra-pulmonary tuberculosis in a case difficult to diagnose conventionally.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Tuberculose dos Linfonodos
/
DNA
/
Prednisolona
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Trop Med Hyg
Ano de publicação:
1994
Tipo de documento:
Article