Flow-cytometric analysis of mepacrine-labelled platelets in patients with end-stage renal failure.
Haemostasis
; 23(5): 284-92, 1993.
Article
em En
| MEDLINE
| ID: mdl-8175050
ABSTRACT
Platelet dysfunction and increased bleeding tendency has been the most consistently described haemostatic abnormality in patients with renal failure. Besides abnormalities in platelet membrane glycoproteins, a reduced amount of platelet-dense granule content has been demonstrated in patients with end-stage renal failure (ESRD) indicating an acquired storage pool deficiency (SPD) present in uraemia. To study dense granules, platelets were labelled with mepacrine, a fluorescent probe which is specifically incorporated into dense bodies. MepaPlatelets of 13 patients with ESRD and of 11 healthy controls were studied. The results showed that mepacrine-labelled platelets of patients with ESRD reveal a significantly (p < 0.05) reduced fluorescence compared to the control group. This implies a reduced number or content of dense granules present in ESRD platelets. Thus, the current data indicate that ESRD is associated with an acquired platelet SPD which may be a useful and rapid method for screening patients with suspected acquired or inherited SPD.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinacrina
/
Plaquetas
/
Deficiência do Pool Plaquetário
/
Grânulos Citoplasmáticos
/
Citometria de Fluxo
/
Falência Renal Crônica
Tipo de estudo:
Etiology_studies
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Haemostasis
Ano de publicação:
1993
Tipo de documento:
Article