Partial purification and characterization of Borna disease virions released from infected neuroblastoma cells.
Virology
; 201(2): 380-2, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-8184547
ABSTRACT
Borna disease is a rare but severe neurological disease of horses and sheep. Borna disease virus (BDV) has not been fully characterized because cell-free virus has not been isolated. Homogenates of infected brain are infectious both for animals and for some cell lines in culture. We report here the partial purification and characterization of cell-free BDV from the tissue culture supernatant of infected human neuroblastoma SKNSH cells. A single negative strand 10-kb RNA was detected in purified virions. Immunoprecipitation analysis of the BDV proteins in purified virions shows the presence of the 60-, 38-, 24-, and 14-kDa proteins previously identified as BDV-specific proteins in infected cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Doença de Borna
Limite:
Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
1994
Tipo de documento:
Article