Changes in translational accuracy of Escherichia coli when folate metabolism is perturbed.
Microbios
; 77(313): 231-7, 1994.
Article
em En
| MEDLINE
| ID: mdl-8208138
ABSTRACT
Translational accuracy was monitored in Escherichia coli mutants which contain abnormal folate pools. A decrease in translational accuracy was indicated by the increased production of a T4 phage mutant containing a UGA mutation in a tail fibre gene. An E. coli folC mutant suppressed the phage mutant under conditions where it presumably accumulated methyl-tetrahydrofolate (methyl-THF). A UGA suppressor strain with the mutation affecting the primary structure of the tRNA(trp) normally suppressed the phage mutant. When the strain was made thymine-requiring it no longer suppressed. The accumulation of methyl-THF which permits suppression by thymine-requiring strains may act to interfere with suppression by the tRNA suppressor, possibly by changing the modification pattern of the tRNA.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Supressão Genética
/
Biossíntese de Proteínas
/
Escherichia coli
/
Ácido Fólico
Idioma:
En
Revista:
Microbios
Ano de publicação:
1994
Tipo de documento:
Article