Stepwise assembly of functionally active transport vesicles.
Cell
; 75(5): 1015-25, 1993 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-8252615
ABSTRACT
Budding of COP-coated vesicles (the likely carriers of newly synthesized proteins from the endoplasmic reticulum through the Golgi stack) from Golgi cisternae requires ADP-ribosylation factor (ARF), coatomer proteins from the cytosol, GTP, and fatty acyl-coenzyme A (CoA). The assembly of coated buds on the membranes requires coatomer, ARF, and GTP. When palmitoyl-CoA is added, membrane fission occurs at the coated bud, releasing coated vesicles. We show that COP-coated vesicles can be generated stepwise in vitro and isolated in a functionally active form, demonstrating that the minimal set of cytosolic components required for their formation as well as principal steps in their assembly have been identified.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Organelas
/
Proteínas de Ligação ao GTP
/
Complexo de Golgi
/
Membranas Intracelulares
/
Proteínas de Membrana
/
Proteínas Associadas aos Microtúbulos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Cell
Ano de publicação:
1993
Tipo de documento:
Article