Histochemical detection of binding sites for human growth hormone using biotinylated ligand.
J Histochem Cytochem
; 42(1): 103-7, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-8263321
ABSTRACT
Recombinant human growth hormone was covalently linked to biotin via a six-carbon spacer arm. Biotinylation was confirmed by electrophoresis and mass spectrometry showed that approximately 50% of the hormone was monobiotinylated. The modified growth hormone (GH) was shown to bind to the GH receptor of IM9 human lymphoid cells with an affinity of 0.55 x 10(9) M-1. Bioactivity of biotinylated GH measured in the Nb2 bioassay was 53.9% that of unlabeled GH. GH binding sites on human IM9 cells were visualized histochemically with the biotinylated hormone, a technique that provides a means of identifying receptors for GH on target cells in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sítios de Ligação
/
Receptores da Somatotropina
/
Hormônio do Crescimento
/
Histocitoquímica
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Histochem Cytochem
Ano de publicação:
1994
Tipo de documento:
Article