Unexpected patterns of Epstein-Barr virus gene expression during early stages of B cell transformation.
Int Immunol
; 5(7): 707-16, 1993 Jul.
Article
em En
| MEDLINE
| ID: mdl-8396414
ABSTRACT
Following Epstein-Barr virus (EBV) binding to its CD21 cell surface receptor, virus internalization, nuclear translocation, and circularization of the viral episome were found to occur within 30 min, immediately preceding the expression of EB nuclear antigen (EBNA)-1 and -2 and latent membrane protein (LMP)-1 and -2 genes. Early viral gene expression was unaffected by blockade of the virus induced, transformation-prerequisite cellular activation pathway (Ca2+ currents, tyrosine phosphorylation, induction of p56lck, hsp70, and hsp90). Despite life times of only 3 h, antisense (but not sense) oligonucleotides for the above latency genes prevented subsequent transformation. Any one antisense oligonucleotide dramatically depleted transcripts not only of the target gene, but of all other latency genes. The blocking effect of antisense oligonucleotides allowed us to identify a new transformation-prerequisite latency gene near the fused termini. The concerted regulation of EBV gene expression is highly unusual and unexplained but our results imply critical, perhaps regulatory roles for initial latency gene transcripts themselves.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Regulação Viral da Expressão Gênica
/
Transformação Celular Viral
/
Herpesvirus Humano 4
Limite:
Child
/
Humans
Idioma:
En
Revista:
Int Immunol
Ano de publicação:
1993
Tipo de documento:
Article