Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis.
J Bacteriol
; 175(2): 565-7, 1993 Jan.
Article
em En
| MEDLINE
| ID: mdl-8419306
ABSTRACT
Escherichia coli alpha-ketoglutarate permease (KgtP) is a 432-amino-acid protein that symports alpha-ketoglutarate and protons. KgtP was predicted to contain 12 membrane-spanning domains on the basis of a calculated hydropathy profile. The membrane topology model of KgtP was analyzed by using kgtP-phoA gene fusions and measuring alkaline phosphatase activities in cells expressing the chimeric proteins. Comparisons of the phosphatase activity levels and the locations of the KgtP-PhoA junctions are consistent with the predicted membrane topology model of KgtP.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Proteínas Recombinantes de Fusão
/
Proteínas de Transporte
/
Estrutura Secundária de Proteína
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Proteínas de Escherichia coli
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Transportadores de Ácidos Dicarboxílicos
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Fosfatase Alcalina
/
Escherichia coli
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1993
Tipo de documento:
Article