A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.
Nat Genet
; 3(1): 44-8, 1993 Jan.
Article
em En
| MEDLINE
| ID: mdl-8490653
ABSTRACT
To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Técnicas Genéticas
/
Proteínas de Ligação a RNA
/
Feto
/
Síndrome do Cromossomo X Frágil
/
Proteínas do Tecido Nervoso
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Nat Genet
Ano de publicação:
1993
Tipo de documento:
Article