Protein A used in DELFIA for the determination of specific antibodies.
Immunol Lett
; 35(3): 285-9, 1993 Mar.
Article
em En
| MEDLINE
| ID: mdl-8514339
ABSTRACT
The conditions of protein A labelling with Eu chelates were studied. The conjugates obtained were compared with those from horseradish peroxidase used conventionally in immunochemical practice. Protein A-Eu conjugates were obtained by a method applied previously for antibody labelling with indium and europium chelates using the bicyclic dianhydride of diethylenetriaminepenta-acetic acid (DADTPA) with some modifications. The Eu-labeled protein A ensured a sensitivity of the IgG determination at the level of 2 ng/ml and a dynamic range of the determination from 3 to 1000 ng/ml, which significantly exceed analogous values for the protein A-peroxidase conjugates. The Eu-labeled protein A was used for the determination of antibodies to Francisella tularensis in the sera of humans vaccinated against tularemia. The assay values exceeded by 10-40-fold the results of an ELISA in sensitivity. It was deduced that the Eu-labeled protein A can be effectively used for the determination of antibodies specific to a tularemia causative agent. In particular, this compound can be useful for the determination of specific antibodies in low immune sera.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteína Estafilocócica A
/
Imunofluorescência
Limite:
Humans
Idioma:
En
Revista:
Immunol Lett
Ano de publicação:
1993
Tipo de documento:
Article