Chemiluminescent and colorimetric enzymatic assays for the detection of PCR-amplified Salmonella sp. products in microplates.
Biotechniques
; 19(5): 792-6, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-8588918
ABSTRACT
To improve Salmonella detection, we developed nonradioactive hybridization assays of amplified products from pure Salmonella cultures. Biotin-labeled PCR products were trapped by internal probes covalently bound to CovaLink-NH MicroWells and detected by colorimetric or chemiluminescent enzymatic reactions. The sensitivities of colorimetric assays using peroxidase and alkaline phosphatase were similar to those obtained with an ethidium bromide-stained agarose gel; both procedures allow the detection of 50 Salmonella cells. Chemiluminescence was 10-fold more sensitive than colorimetry.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Salmonella
/
DNA Bacteriano
/
Reação em Cadeia da Polimerase
/
Colorimetria
/
Medições Luminescentes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1995
Tipo de documento:
Article