Intracellular free Ca2+ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells.
FEBS Lett
; 386(2-3): 123-7, 1996 May 20.
Article
em En
| MEDLINE
| ID: mdl-8647265
ABSTRACT
This study documents a difference between cystic fibrosis human (CF-HTG) and normal human (HTG) tracheal gland cells the ability of histamine to induce an increase of intracellular free calcium concentration [Ca2+]i was abnormally reduced in CF-HTG cells. The magnitude of the [Ca2+]i peak rise in response to histamine is smaller in CF-HTG cells than in HTG cells, and the percentage of CF-HTG cells that increase [Ca2+]i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF-HTG and HTG cells generated [Ca2+]i asynchronous oscillations and the magnitude of the peak [Ca2+]i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF-HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca2+]i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traqueia
/
Histamina
/
Cálcio
/
Fibrose Cística
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article