Galantide distinguishes putative subtypes of galanin receptors in mudpuppy parasympathetic neurons.
Eur J Pharmacol
; 287(1): 97-100, 1995 Dec 04.
Article
em En
| MEDLINE
| ID: mdl-8666034
ABSTRACT
The effect of the chimeric ligand galantide on the galanin-induced activation of membrane K+ conductance and inhibition of voltage-dependent Ca2+ conductance has been studied using voltage-clamped dissociated mudpuppy parasympathetic neurons. Galantide did not activate the K+ conductance but produced a concentration-dependent antagonism (IC50 = 4 nM) of the galanin-induced increase in K+ conductance. Galantide acted like galanin and inhibited the voltage-dependent Ba2+ current (IBa). The inhibition of IBa also was concentration dependent (IC50 = 16 nM) and the maximum inhibition produced by galantide was approximately 40%. We also demonstrate that the galanin-(1-16) fragment increased the membrane K+ conductance and decreased IBa, suggesting that the NH2 portion of the galanin molecule is sufficient to mediate both actions. One interpretation of these observations is that different galanin receptors mediate the different effects of galanin on the mudpuppy parasympathetic neurons.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema Nervoso Parassimpático
/
Receptores dos Hormônios Gastrointestinais
/
Substância P
/
Galanina
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Eur J Pharmacol
Ano de publicação:
1995
Tipo de documento:
Article