BSTI, a trypsin inhibitor from skin secretions of Bombina bombina related to protease inhibitors of nematodes.
Protein Sci
; 5(2): 357-62, 1996 Feb.
Article
em En
| MEDLINE
| ID: mdl-8745414
ABSTRACT
From skin secretions of the European frog Bombina bombina, a new peptide has been isolated that contains 60 amino acids, including 10 cysteine residues. Its sequence was determined by automated Edman degradation and confirmed by analysis of the cDNA encoding the precursor. A search in the databanks demonstrated that the pattern of cysteine residues in this skin peptide is similar to the ones found in protease inhibitors from Ascaris and in a segment of human von Willebrand factor. The 3D structure of the trypsin inhibitor from Ascaris suum could be used as a template to build a model of the amphibian peptide. In addition, we have demonstrated that this constituent of skin secretion is indeed an inhibitor of trypsin and thrombin, with K(i) values in the range of 0.1 to 1 microM. The new peptide was thus named BSTI for Bombina skin trypsin/thrombin inhibitor.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Anuros
/
Inibidores de Proteases
/
Conformação Proteica
/
Ascaris
/
Pele
/
Proteínas
/
Modelos Moleculares
/
Proteínas de Helminto
/
Inibidores da Tripsina
/
Antitrombinas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Protein Sci
Ano de publicação:
1996
Tipo de documento:
Article