Crystallization and preliminary X-ray crystallographic analysis of ImmE7 protein of colicin E7.
Proteins
; 23(4): 588-90, 1995 Dec.
Article
em En
| MEDLINE
| ID: mdl-8749855
ABSTRACT
The ImmE7 protein, which can bind specifically to the DNase colicin E7 and neutralize its bactericidal activity, has been purified and crystallized in two different crystal forms by vapor diffusion method. The orthorhombic crystals belong to space group I222 or I2(1)2(1)2(1) and have unit cell dimensions a = 75.1 A, b = 50.5 A, and c = 45.4 A. The second form is monoclinic space group P2(1) with cell dimensions a = 29.3 A, b = 102.7 A, c = 53.0 A, and beta = 91.5 degrees. The orthorhombic crystals diffract to 1.8 A resolution, and are suitable for high-resolution X-ray analysis.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Proteínas de Bactérias
/
Colicinas
Idioma:
En
Revista:
Proteins
Ano de publicação:
1995
Tipo de documento:
Article