Heterologous expression of sarcoplasmic reticulum Ca(2+)-ATPase.
Biosci Rep
; 16(2): 107-13, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8790916
ABSTRACT
In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca(2+)-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retículo Sarcoplasmático
/
ATPases Transportadoras de Cálcio
Limite:
Animals
Idioma:
En
Revista:
Biosci Rep
Ano de publicação:
1996
Tipo de documento:
Article