Isolation of two strains (S-R type) of Bacillus circulans and purification of a cyclomaltodextrin-glucanotransferase.
Cell Mol Biol (Noisy-le-grand)
; 42(5): 653-7, 1996 Jul.
Article
em En
| MEDLINE
| ID: mdl-8832095
ABSTRACT
From wild type inoculum of Bacillus circulans DF 9 which produce cyclomaltodextrin-glucanotransferase (EC 2.4.1.19, CGTase), two different kinds of colonies were isolated, which correspond to the classical S-R variation. From the culture medium of both colonies grown together a CGTase was purified about 50 fold with a yield of 54% in two steps. From pure R-cell culture the enzyme was purified by about 38 folds with a yield of 79% in only one step, showing a complete homogeneity as judged by a native PAGE analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus
/
Glucosiltransferases
Idioma:
En
Revista:
Cell Mol Biol (Noisy-le-grand)
Ano de publicação:
1996
Tipo de documento:
Article