Protein topology of presenilin 1.
Neuron
; 17(5): 1023-30, 1996 Nov.
Article
em En
| MEDLINE
| ID: mdl-8938133
ABSTRACT
Mutations in a gene encoding a multitransmembrane protein, termed presenilin 1 (PS1), are causative in the majority of early-onset cases of AD. To determine the topology of PS1, we utilized two strategies first, we tested whether putative transmembranes are sufficient to export a protease-sensitive substrate across a lipid bilayer; and second, we examined the binding of antibodies to specific PS1 epitopes in cultured cells selectively permeabilized with the pore-forming toxin, streptolysin-O. We document that the "loop," N-terminal, and C-terminal domains of PS1 are oriented toward the cytoplasm.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Neuron
Ano de publicação:
1996
Tipo de documento:
Article