Metabolic fate of an oral tracer dose of [13C]docosahexaenoic acid triglycerides in the rat.
Am J Physiol
; 270(4 Pt 2): R846-54, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8967415
ABSTRACT
The appearance of 13C in rat lipoprotein, blood cells, and brain lipids was followed as a function of time after the ingestion of triglycerides (TG) containing [13C]226n-3. The time course of 13C abundance in 226n-3 of various lipid pools, measured by gas chromatography combustion-isotope mass spectrometry, established precursor-product relationships within lipids. The [13C]226n-3 was rapidly incorporated into very low density lipoprotein-chylomicron-TG and unesterified fatty acids bound to albumin, with a concomitant maximal appearance at 3 h and further decline. Lysophosphatidylcholines (lysoPC) bound to albumin were also enriched in [13C]226n-3, and their labeling appeared to be mainly due to hepatic secretion at the earliest time points. From 12 h postingestion, the synthesis of [13C]226n-3-lysoPC was twice as high as that of unesterified [13C]226n-3, making lysoPC a potential source of 226n-3 supply for tissues. The labeling of platelets, red blood cells, and brain phospholipids presented different kinetics, presumably involving the two lipid forms of [13C]226n-3 bound to albumin, to different extents. We conclude that [13C]226n-3 esterified in TG is rapidly redistributed within blood lipoproteins and the albumin fraction and that its incorporation in lipid species bound to albumin influences its uptake by target tissues.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triglicerídeos
/
Ácidos Docosa-Hexaenoicos
Limite:
Animals
Idioma:
En
Revista:
Am J Physiol
Ano de publicação:
1996
Tipo de documento:
Article