Analysis of mesocarb analogues in urine and plasma of rats by high-performance liquid chromatography and thermospray liquid chromatography-mass spectrometry.
J Chromatogr B Biomed Appl
; 687(1): 261-9, 1996 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-9001972
ABSTRACT
The pharmacokinetics and metabolism of synthetic 2-hydroxymesocarb and 4-methyl-2-hydroxymesocarb analyzed by HPLC-DAD and thermospray LC-MS were studied in rats. Multistep liquid-liquid extraction (LLE) was used with diethyl ether at pH 7.0. The major metabolites of 2-hydroxymesocarb in both urine and plasma of the rat were the p-hydroxylated derivative of the phenylcarbamoyl group of the parent drug. The metabolites of 4-methyl-2-hydroxymesocarb in urine of rats may be the oxidized forms at the phenylcarbamoyl group of the parent drug. Absorption (ka) and elimination (ke) rate constants in plasma of 2-hydroxy-mesocarb were 0.0300 and 0.00485 min-1, respectively; those of 4-methyl-2-hydroxymesocarb were 0.0546 and 0.00797 min-1, respectively. The half-lives (t1/2) of 2-hydroxymesocarb and 4-methyl-2-hydroxymesocarb in plasma were 144 and 86 min, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sidnonas
/
Cromatografia Líquida de Alta Pressão
/
Estimulantes do Sistema Nervoso Central
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Biomed Appl
Ano de publicação:
1996
Tipo de documento:
Article