Effects of selenium and serum on selenoprotein W in cultured L8 muscle cells.
Biometals
; 10(1): 11-22, 1997 Jan.
Article
em En
| MEDLINE
| ID: mdl-9002179
ABSTRACT
When rat L8 muscle cells were cultured to examine the effects of serum and selenium concentration on selenoprotein W levels and glutathione peroxidase (GPX) activities, no significant differences (P > 0.05) were found in selenoprotein W levels and GPX activities during differentiation. With three different forms of selenium, selenoprotein W levels and GPX activities were shown to increase in L8 myotubes cultured in media with these selenocompounds. Selenite was utilized more efficiently than selenocysteine for both selenoprotein W and GPX activity, but selenium as selenomethionine was less available. Both the protein content and mRNA levels for selenoprotein W were affected by the selenium content of the media. Northern blot data indicated that the expression of selenoprotein W mRNA increased significantly when L8 myotubes were cultured with selenium (P > 0.05). L8 myotubes cultured in 10% calf serum (CS) versus 2% CS with or without addition of 10(-8) M selenium indicated that the increase of selenoprotein W level in L8 myotubes cultured with higher serum concentration (10% CS) is due to the higher selenium concentration in media rather than serum itself.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Selênio
/
Fenômenos Fisiológicos Sanguíneos
/
Proteínas
/
Músculo Esquelético
Limite:
Animals
Idioma:
En
Revista:
Biometals
Ano de publicação:
1997
Tipo de documento:
Article